| Column | Content | Required? | Example |
| 1 | DB | required | SGD |
| 2 | DB_Object_ID | required | S000000296 |
| 3 | DB_Object_Symbol | required | PHO3 |
| 4 | Qualifier | optional | NOT |
| 5 | REF_ID | required | GO:0003993 |
| 6 | DB:Reference (|DB:Reference) | required | PMID:2676709 |
| 7 | Evidence code | required | IMP |
| 8 | With (or) From | optional | GO:0000346 |
| 9 | Aspect | required | F |
| 10 | DB_Object_Name | optional | acid phosphatase |
| 11 | DB_Object_Synonym (|Synonym) | optional | YBR092C |
| 12 | DB_Object_Type | required | gene |
| 13 | taxon(|taxon) | required | taxon:4932 |
| 14 | Date | required | 20010118 |
| 15 | Assigned_by | required | SGD |